New tool: localres
I just finished putting together a command-line tool to plot a histogram of
local resolution values from a local resolution 3D map and a 3D mask
mask.mrc files from RELION, respectively). This is a
useful visualization to complement a color-coded local resolution scale mapped
onto the 3D reconstruction (or visualized across slices of said reconstruction)
as usually shown in publications describing a cryo-EM structure. An example is
displayed in Figure S3C of my recent preprint.
I got the idea from a Twitter conversation, reused the suggested code and wrapped it in a command-line tool that is documented and easy to install. The (very simple) code is available here. I also made it citeable with doi:10.5281/zenodo.3575230 .
This was a good toy project to learn some basics of Python, especially to learn how to use the click library to parse command-line arguments, how to package a Python program and how to distribute it. I was surprised that anyone can upload anything to the Python Package Index without any form of review (in the R world, a review team checks every package submitted to the CRAN repository).